AAV, LV, and AdV virus packaging, library construction, and cell line development, providing ready-to-use, reliable tools to accelerate gene research and functional studies.

Virus Packaging

Overview

Virus packaging services offer high-quality, ready-to-use viral vectors for gene delivery, functional genomics, gene therapy studies, and CRISPR or shRNA library experiments. Our services cover a comprehensive suite of solutions for genetic research, including Adeno-Associated Virus (AAV) packaging, Lentivirus (LV) packaging, Adenovirus (AdV) packaging, library construction, and cell line construction. Our viral vectors are produced under strict quality control, ensuring high titer, purity, and safety, while offering fast production, reproducibility, scalability, and endotoxin-free preparations. Combined with expert support for custom library generation and reliable cell line construction, we provide end-to-end solutions that streamline your experiments, accelerate research workflows, and allow you to focus on scientific discovery.

Key Advantages of Virus Packaging

High Quality and Safety

Viral vectors are produced under strict quality control with high titer, purity, and endotoxin-free preparations.

Fast Turnaround

Gene synthesis and viral vector packaging delivered in as fast as 15 days.

Comprehensive Solutions

Coverage of AAV, LV, and AdV packaging, plus customized library construction and stable cell line generation for diverse applications.

Research Acceleration

Ready-to-use vectors and expert technical support streamline experimental workflows, enabling faster functional genomics and gene therapy studies.

Service Details of Virus Packaging

Workflow of Virus Packaging

Sequence Design & Synthesis: Design and synthesize the DNA sequence (gene or sgRNA cassette) of interest.

Plasmid Preparation: Clone the gene or sgRNA cassette into an appropriate transfer plasmid and prepare high-quality DNA.

Virus Packaging: Introduce plasmids into producer cells to generate viral particles.

Centrifugation / Purification: Clarify, concentrate, and purify the viral supernatant.

Quality Control & Delivery: Measure titer, verify purity, and provide the virus ready for use along with documentation.

Application of Virus Packaging

Gene Delivery & Expression

Efficient, high-titer viral vectors for stable or transient gene delivery in cells.

Functional Genomics & Screening

CRISPR, sgRNA, or mutagenesis libraries for genome-wide or pathway-specific studies.

Disease Modeling & Experimental Research

Stable cell lines and in vivo models to study gene function and therapy.

FAQs of Virus Packaging

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Do I need to prepare my own plasmids?

No. Our service includes plasmid preparation, where we clone the gene or sgRNA cassette into an appropriate transfer plasmid and prepare high-quality DNA.

Can I get a guarantee service for virus packaging?

Yes. For each category of viruses (AAV, ADV, lentivirus, etc.), clients can opt for a guarantee service to ensure the viral vectors meet expected performance standards.

Can you help me generate stable cell lines?

Yes. We offer custom stable cell line construction, including knockout, overexpression/interference, point mutation, and knock-in cell lines.

Delivered as frozen or cultured cells (≥1×10⁶ cells per vial).

Turnaround time: 6–11 weeks depending on cell type.

Quality verification: Sanger sequencing, qPCR, or protein-level validation.

Includes positive clones and, when applicable, control lines ready for downstream experiments.

Leading Biotech Innovation with AI-Powered, End-to-End Platform for DNA/RNA Synthesis, from Raw Materials to Industrial-Scale Functional Molecule Production.

Core Capabilities of Tsingke Gene Factory

Tsingke Gene Factory is at the heart of Tsingke Biotech’s strategic vision, offering a fully integrated platform for DNA/RNA synthesis. Our mission, "Biotech for a Better World," drives us to empower global scientific discovery and biopharmaceutical innovation.

With expertise in Gene Reading, Writing, and Editing, we have built an independent R&D ecosystem that covers the entire value chain—from raw material production and instrument manufacturing to advanced synthesis workflows. This comprehensive ecosystem enables us to provide end-to-end solutions, spanning from raw materials to sequencing.

Our AI-powered platform further enhances this ecosystem by incorporating high-throughput oligonucleotide manufacturing, antibody discovery and production, as well as strain engineering and screening. With in-house reagents, self-developed synthesizers, and automation systems, the factory is built for scalability, speed, and precision.

Capable of supporting production from microgram-level to industrial scale, we ensure the high quality standards across biopharmaceuticals, agriculture, and other industries worldwide.

End-to-End Integration

Raw Material Production: DNA/RNA phosphoramidites, solid supports, and synthesis reagents produced in-house.

Instrument Development: Proprietary oligo synthesizers (12P, 192B, 768B), purifiers, and automation platforms.

Manufacturing Workflow: AI-driven production management system (Tsingke HelixTech) enabling real-time tracking, quality control, and lean manufacturing.

Synthesis Excellence

Tsynth® gene synthesis: Up to 200 kb in length with 200+ ready-to-use vectors, free codon optimization, and cloning services.

Oligo & Modified Nucleic Acids: Over 200 modification types, multiple purification options, and support for research, diagnostics, and therapeutics.

RNA Synthesis: mRNA, saRNA, circRNA with LNP formulation services.

Protein & Antibody Services: From gene to protein expression and purification.

Quality and Compliance

Certifications: Tsingke operates an ISO 13485-certified manufacturing facility.

Validation: Full NGS verification, QC traceability, and strict SOP management.

Applications of Tsingke Gene Factory

Molecular Diagnostics

qPCR probes, hybridization probes, detection primers.

Vaccine Development

mRNA synthesis, LNP delivery, protein antigens, viral vectors.

Gene Editing

ssODN, gRNA/CRISPR tools, expression vectors.

Gene Therapy

GMP-ready plasmids, virus packaging, saRNA therapeutics.

Agricultural Biotech

Pathogen detection, veterinary probes, trait engineering constructs.

Antibody Drug Discovery

Custom Gene Synthesis，Synthetic Antibody Librarie，Stable Cell Line Support

Why Choose Tsingke Gene Factory

Fully Vertical Supply Chain

From monomers to final product, all in-house for maximum quality control.

Automation & AI

Streamlined workflows for faster turnaround and fewer errors.

Scalable Production

From research-scale to industrial GMP batches.

Proven Track Record

Trusted by 300,000+ researchers worldwide.

Global Vision

Serving academia, diagnostics, and biotech companies across 100+ count

High-purity single- and double-stranded AAV vectors with tailored titers, supporting overexpression, knockdown (shRNA), and gene knockout (CRISPR-SaCas9) for reliable experimental results.

Highlights of AAV Packaging Service

Adeno-associated virus (AAV) is a uniquely versatile vector, valued for its ability to efficiently transduce a wide range of mammalian cells while being naturally non-pathogenic in humans and exhibiting low immunogenicity. Our custom AAV packaging services deliver high-purity single-and double-stranded vectors optimized for overexpression, shRNA-mediated knockdown, and CRISPR-SaCas9 gene editing. Leveraging proprietary technologies and reagents, we ensure superior viral titers, purity, potency, and consistency for reliable and reproducible experimental outcomes. Flexible packaging options accommodate both standard and ultra-low yield serotypes. Trusted by researchers worldwide, our AAV vectors accelerate gene function studies, streamline workflows, and support advanced functional genomics projects.

Cases of AAV Packaging

In Vitro Applications: Tsingke AAV viruses of different serotypes show good infection efficiency across various cell types.

FAQs of AAV Packaging

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The target cells can be transduced by AAV, but why is the GFP fluorescence intensity very low?

The GFP fluorescence intensity in target cells depends on several factors, including the number of viral particles transducing the cells, the proliferative state of the cells, the cell type, and the observation time. Generally, the higher the number of viral particles transducing the target cells, and the faster the cell proliferation, the stronger the GFP fluorescence. For cells with faster proliferation, GFP expression typically peaks 48 to 72 hours after viral transduction. For slower proliferating cells, the GFP expression time will be extended.

The transduction efficiency of AAV in target cells is low. How can the virus transduction efficiency be improved?

At Tsingke, we generally improve transduction efficiency by increasing the MOI (multiplicity of infection). Additionally, maintaining the target cells in good growth condition is crucial to ensure normal transduction efficiency.

What is the packaging capacity of AAV viruses?

AAV can package approximately 3.0 kb of genetic material. However, if you want to add a marker, such as GFP or RFP, the size of the gene that can be inserted will be smaller, around 1.5 kb.

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Custom DNA & RNA Oligo Synthesis – Fast, Flexible, Full-Stack From short DNA primers to complex RNA therapeutics, Tsingke provides high-quality oligos with full customization and ISO-certified QC.

Oligo Synthesis Overview

Tsingke provides high-quality DNA and RNA oligonucleotides synthesized with self-developed phosphoramidites and automation platforms. Our service supports complex modifications, scalable throughput, and stringent QC options—ideal for diagnostics, genome editing, sequencing, and synthetic biology applications.

Custom DNA Oligo

Short primers, probes, and NGS adapters for research, diagnostics, and gene editing.

Custom RNA Oligo

Custom RNA, siRNA, miRNA, sgRNA and ASO for gene editing, functional research, and nucleic acid drugs.

Oligo Modifications

Fluorophores, quenchers, modified bases, linkers, and spacers, for qPCR, sequencing, antisense therapeutics, and probe design.

Resources of Tsingke Oligo Synthesis

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Tsingke Oligo Synthesis Brochure 1.2.1.250805

Tsingke piRNA Synthesis Flyer 1.1.1.250724

Tsingke RNA Synthesis Flyer 1.1.1.240826

Tsingke RNA Order 1.1.1.250815

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Tsingke DNA Order 1.1.1.250815

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FAQs of Oligo Synthesis

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What is the principle of oligo synthesis and what types of services are offered?

Oligo synthesis utilizes solid-phase phosphoramidite chemistry.We synthesize a wide range of oligonucleotides, including:

(1) DNA oligos;

(2) RNA oligos;

(3) Probes;

(4) siRNA;

(5) sgRNA;

(6) miRNA;

(7) ASOs;

(8) Various modified oligos.

What contaminants are present in the crude product?

Crude oligonucleotides contain the desired product, failure sequences and chemically modified by-products.

How to store oligonucleotides?

(1) Storage (Freeze-Dried): oligonucleotide primers in a freeze-dried state are highly stable and can be stored at -20°C for at least one year.

(2) Storage (Dissolved): Dissolved oligonucleotides can be stored as a 100 μM stock solution, aliquoted into multiple tubes, and kept at -20°C for at least six months (frequent freeze-thaw cycles may reduce shelf life).

Before use: Dilute the concentrated stock solution to the working concentration required for your experiments.

How to dissolve oligonucleotides?

Before opening the vial, centrifuge it at 3000–4000 rpm for 1 minute to ensure the powder is at the bottom and prevent loss.

Add an appropriate amount of nuclease-free double-distilled water (pH > 6.0) or TE buffer (pH 7.5–8.0) to achieve a 100 μM stock solution for each OD.

Why do resuspended primers perform well initially but degrade after storage?

Primer degradation occurs if the dissolving water has a low pH or is contaminated with bacteria or nucleases. Inadequate thawing and mixing when using the aliquot may also lead to inaccurate primer addition.

Recommendation:

* Aliquot the primers to avoid repeated freeze-thawing.

* Use 10 mMTris buffer (pH 7.5) to dissolve the primers.

* Note that sometimes the primer itself is not faulty, but the quality of other PCR materials (especially the template) may not be the same as previously used.

If you want to know more about oligonucleotide synthesis service, please visit our website.